gluten free diet/probiotics

[kim]

Seems this could have something to do with an individuals response to a gluten free diet and probiotics. I'm wondering if those who mount an abnormally high antibody response would see a negative response more often? I know the "strep strains" in probiotics have been the suspected culprit when a child doesn't seem to do well on probiotics but this might be another thing to consider.

 

bolding mine

 

*1: Br J Nutr. 2009 May 18:1-7. [Epub ahead of print]

Click here to read

<http://www.ncbi. nlm.nih.gov/ entrez/utils/ fref.fcgi? PrId=3288& itool=Abstract- def&uid=19445821 &db=pubmed& url=http: //journals. cambridge. org/abstract_ S000711450937176 7>

 

*Effects of a gluten-free diet on gut microbiota and immune function in

healthy adult human subjects.*

 

De Palma G, Nadal I, Collado MC, Sanz Y.

 

Microbial Ecophysiology and Nutrition Group, Institute of Agrochemistry

and Food Technology (IATA), Spanish National Research Council (CSIC), PO

Box 73, 46100 Burjassot, Valencia, Spain.

 

Diet influences the composition of the gut microbiota and host's health,

particularly in patients suffering from food-related diseases. Coeliac

disease (CD) is a permanent intolerance to cereal gluten proteins and

the only therapy for the patients is to adhere to a life-long

gluten-free diet (GFD). In the present preliminary study, the effects of

a GFD on the composition and immune function of the gut microbiota were

analysed in ten healthy subjects (mean age 30.3 years) over 1 month.

Faecal microbiota was analysed by fluorescence in situ hybridisation

(FISH) and quantitative PCR (qPCR). The ability of faecal bacteria to

stimulate cytokine production by peripheral blood mononuclear cells

(PBMC) was determined by ELISA. No significant differences in dietary

intake were found before and after the GFD except for reductions (P =

0.001) in polysaccharides. Bifidobacterium, Clostridium lituseburense

and Faecalibacterium prausnitzii proportions decreased (P = 0.007, P =

0.031 and P = 0.009, respectively) as a result of the GFD analysed by

FISH. Bifidobacterium, Lactobacillus and Bifidobacterium longum counts

decreased (P = 0.020, P = 0.001 and P = 0.017, respectively) , while

Enterobacteriaceae and Escherichia coli counts increased (P = 0.005 and

P = 0.003) after the GFD assessed by qPCR. TNF-alpha, interferon-gamma,

IL-10 and IL-8 production by PBMC stimulated with faecal samples was

also reduced (P = 0.021, P = 0.037, P = 0.002 and P = 0.007,

respectively) after the diet. Therefore, the GFD led to reductions in

beneficial gut bacteria populations and the ability of faecal samples to

stimulate the host's immunity. Thus, the GFD may constitute an

environmental variable to be considered in treated CD patients for its

possible effects on gut health.

[pmoreno]

Seems this could have something to do with an individuals response to a gluten free diet and probiotics. I'm wondering if those who mount an abnormally high antibody response would see a negative response more often? I know the "strep strains" in probiotics have been the suspected culprit when a child doesn't seem to do well on probiotics but this might be another thing to consider.

 

bolding mine

 

*1: Br J Nutr. 2009 May 18:1-7. [Epub ahead of print]

Click here to read

<http://www.ncbi. nlm.nih.gov/ entrez/utils/ fref.fcgi? PrId=3288& itool=Abstract- def&uid=19445821 &db=pubmed& url=http: //journals. cambridge. org/abstract_ S000711450937176 7>

 

*Effects of a gluten-free diet on gut microbiota and immune function in

healthy adult human subjects.*

 

De Palma G, Nadal I, Collado MC, Sanz Y.

 

Microbial Ecophysiology and Nutrition Group, Institute of Agrochemistry

and Food Technology (IATA), Spanish National Research Council (CSIC), PO

Box 73, 46100 Burjassot, Valencia, Spain.

 

Diet influences the composition of the gut microbiota and host's health,

particularly in patients suffering from food-related diseases. Coeliac

disease (CD) is a permanent intolerance to cereal gluten proteins and

the only therapy for the patients is to adhere to a life-long

gluten-free diet (GFD). In the present preliminary study, the effects of

a GFD on the composition and immune function of the gut microbiota were

analysed in ten healthy subjects (mean age 30.3 years) over 1 month.

Faecal microbiota was analysed by fluorescence in situ hybridisation

(FISH) and quantitative PCR (qPCR). The ability of faecal bacteria to

stimulate cytokine production by peripheral blood mononuclear cells

(PBMC) was determined by ELISA. No significant differences in dietary

intake were found before and after the GFD except for reductions (P =

0.001) in polysaccharides. Bifidobacterium, Clostridium lituseburense

and Faecalibacterium prausnitzii proportions decreased (P = 0.007, P =

0.031 and P = 0.009, respectively) as a result of the GFD analysed by

FISH. Bifidobacterium, Lactobacillus and Bifidobacterium longum counts

decreased (P = 0.020, P = 0.001 and P = 0.017, respectively) , while

Enterobacteriaceae and Escherichia coli counts increased (P = 0.005 and

P = 0.003) after the GFD assessed by qPCR. TNF-alpha, interferon-gamma,

IL-10 and IL-8 production by PBMC stimulated with faecal samples was

also reduced (P = 0.021, P = 0.037, P = 0.002 and P = 0.007,

respectively) after the diet. Therefore, the GFD led to reductions in

beneficial gut bacteria populations and the ability of faecal samples to

stimulate the host's immunity. Thus, the GFD may constitute an

environmental variable to be considered in treated CD patients for its

possible effects on gut health.

 

I'm not sure I understand this correctly - Is it saying that by being on a GFD, it cn actually decrease beneficial gut bacteria? Well, that wouldn't be a good thing. Then does it go on to say that it leads to the reduction of ability to stimulate the host's immunity? I'm just having a hard time following this train of thought. Did you read the whole article and have a better understanding? thanks. Pat

[Caryn]

Kim,

This study is buzzing around all the celiac groups today. Most Celiac folks aren't putting much stock in it because they claim the study was done on 'healthy' folks. Celiacs aren't 'healthy' to begin with.

I read another study that showed that Bifidus L. supplementation allowed Celiacs to avoid intestinal damage from gluten ingestion. But all will tell you that the nasty effects of eating wheat far outweigh any study that would tell them that they shouldn't because it reduces the good bacteria in their gut.

 

Interesting study. I question what they defined as a 'gluten free' diet. We aren't grainless here, but are low grain. I think that the gluten free only diet is just as bad in many ways because of the sugar, starch, yeast loving foods that it contains. So, yes, I can see how a packaged food diet that is gluten free can show this kind of result. I would like to see a study like this using the paleo diet instead. I bet the results are totally different.

 

It's all in the poop. If it's healthy then you know you're on the right track.

 

Bad pun, I know.

[kim]

I'm not sure I understand this correctly - Is it saying that by being on a GFD, it cn actually decrease beneficial gut bacteria? Well, that wouldn't be a good thing. Then does it go on to say that it leads to the reduction of ability to stimulate the host's immunity? I'm just having a hard time following this train of thought. Did you read the whole article and have a better understanding? thanks. Pat

Pat,

 

I could relate to your confusion there. I think how you view these findings lies in whether you believe there is a problem with the immune system not responding to an infection or whether the problem is in the "shutting down," of that response. In my own case, I think my problem was in the shut down (and the fact that I let an infection get out of control because I was too stupid to get on an antibiotic quicker). This study says that TNF-alpha, interferon-gamma, IL-10 and IL-8 production by PBMC stimulated with faecal samples was reduced. TNFa was something that I'm sure needed to be reduced in my case! My understanding is that IL10 is sort of a regulator and I would have killed for a hit of that! I'm thinking IL I0 may have been reduced simply because TNFa was? Anyway, this study gives me a little push to reduce grains myself. Another question that I had, was the immune functioning changed directly in relationship to the flora change? Bifido is a strain that seems to be highly recommended. After reading this, I'm confused too, whether the best course of action would be to avoid grains, or avoid grains and supplement with probiotics.

 

Caryn,

 

I thought about the "healthy" individuals too. It was great to see some actual validation regarding bacteria strains (and grain avoidance without apparent celiac "gene" involvement) and immune functioning tho. I'm more than willing to let go of the idea that a certain strain of bacteria might not be something that should be promoted in "all" if that's how this turns out. If you run into other info on this subject, would love to hear it!

 

edit

 

I read another study that showed that Bifidus L. supplementation allowed Celiacs to avoid intestinal damage from gluten ingestion

 

I forgot about that comment. Do you have that article handy?

[kim]

Comments on this study by Teresa Binstock

that i found in my email last nite

 

 

 

* * * * * * * * * * * * * * * * * * * * * *

Teresa Binstock

Researcher in Developmental & Behavioral Neuroanatomy

June 7, 2009

 

 

This post may be forwarded hither and yon.

 

I was quite excited to read a study reporting the intestinal

inflammation induced CNS inflammation and increased CNS excitability -

and these effects via TNFa, which is muchly documented as atypical in

many autistic children. Furthermore, there are ramifications regarding

why GF and/or CF helps many but not all autistic children. The

mini-essay and its citations can be enjoyed at:

 

*/Gluten-free and casein-free diets for subgroups of autistic children/*

http://www.generationrescue.org/binstock/0...c-children.html

 

Teresa Binstock

Researcher in Developmental & Behavioral Neuroanatomy

June 7, 2009

 

Introduction: A recently published study links intestinal inflammation

to an increased tendency for excessive excitation within the CNS (17).

This very important study is free online. Note that the cytokine Tumor

Necrosis Factor alpha (TNFa) is etiologically significant in this

gut-brain connection and that TNFa has been found to be atypical in many

autistic children. Additional relevance of the relationship between

peripheral inflammation and CNS excitability (17) derives from the facts

(a) that casein induces TNFa in children with hypersensitivity to milk,

and ( the gluten-free and casein-free diets have been found to lower TNFa.

 

More, including citations, at:

http://www.generationrescue.org/binstock/0...c-children.html