Looking for help in reading IgeneX results

[colleendonny]

Hello all. We just got back DD's IgeneX results. I have no idea what they mean until we see Dr.B the end of February.

Does anyone know what the following mean?

 

IgM IND on band 39

IgM IND on band 41

IgG IND on band 31

IgG IND on band 39

IgG positive + on band 41

 

Thanks everyone

[KaraM]

Hi Colleen,

 

I am going to quote a handout I have from Dr. J.

 

"There are eight know Borrelia burgdorferi genus specie specific KDA Western Blot antibodies(band): 18, 23, 30, 31, 34, 39, 83 and 93. Only oneof these Borrelia burgdorrferi geus specie specific bands is neededto cnfirm that there is serlogical evidence of exposure to the Borrelia burgdorferi spirochete and can confirm a clinical diagnosisof Lyme disease."

 

If an antibody is genus specie specific, I believe that means that the only known bacteria that causes the response is the Borrelia burgdorferi (i.e the bacteria that causes Lyme). Some other bands can be cross reactive and respond to other bacteria (not specific). (Someone correct me if I have this wrong please).

 

 

As far as understand, the IND means that something showed up on those bands, not quite enough to put it clearly in the positive zone, but more than the nothing that would make it negative... (Someone on this board described it before as the difference between a ball point pen line and a magic marker line). Since the resuts are showing "something" on a "specific band" my guess is that Dr. B. will probably refer you out to an LLMD (mostl likely Dr. J.). I believe you may have already scheduled something with an LLMD...if so, you are one step ahead.

 

 

The CDC's criteria for a positive requires more than one band and not all of those bands are specific...As you learn more about this all, you start to understand that their criteria really does't make sense. As you learn the whole political history behind Lymeandhow these criteria were established, you start to get frustrated and even angry.

 

You may want to get the book, Cure Uknown by Pamela Weintraub and/or watch the documentary Under Our Skin.

 

I am also posting a link that I think LLM put on here a while ago to a pretty good article that summarizes pediatric Lyme. http://content.yudu.com/Library/A1nyhm/NaturalNutmegMagazin/resources/15.htm

 

 

 

Kara

 

 

 

 

 

 

Hello all. We just got back DD's IgeneX results. I have no idea what they mean until we see Dr.B the end of February.

Does anyone know what the following mean?

 

IgM IND on band 39

IgM IND on band 41

IgG IND on band 31

IgG IND on band 39

IgG positive + on band 41

 

Thanks everyone

[colleendonny]

Thanks Kara,

I agree that her testing does look suspicious. Thanks for posting back. I have an appointment with 2 LLMD's. I just need to choose one.

Did you get my email last week? Was wondering of you had a meeting set up for this or next month?

[Lymommyof3]

Hello all. We just got back DD's IgeneX results. I have no idea what they mean until we see Dr.B the end of February.

Does anyone know what the following mean?

 

IgM IND on band 39

IgM IND on band 41

IgG IND on band 31

IgG IND on band 39

IgG positive + on band 41

 

Thanks everyone

 

 

Hi,

I copied this from somewhere else but it helped me understand my kids results.

MEANING OF IGENEX TEST:

 

The following is a breakdown of the Western blot only. As co-infections will show their own titers. A positive IgM is a sign of a current/active infection. IgG can be a sign of an older/longer standing infection and if symptomatic treatment is needed. 

 

 There are nine known Borrelia burgdorferi genus specie specific KDA Western Blot antibodies (bands): 18 23 30 31 34 37 39 83 and 93. Only one of these Borrelia burgdorferi genus specie specific bands is needed to confirm that there is serological evidence of exposure to the Borrelia burgdorferi spirochete and can confirm a clinical diagnosis of Lyme disease. (More info pages 12 & 13 "Western Blot Made Easy": http://www.publichealthalert.org/pdfnew/2007_06.pdf 

~~~~~~~~~~~~~~~~~~~~~~~

 

Igenex Western Blot Break Down by band

9 cross-reactive for Borrellia

12 specific for Bb

18 highly specific to Lyme (Many LLMD's say if this band alone is positive, you have lyme - see link above)

20 cross-reactive for Borrellia

21 unknown

22 specific for Bb, probably really the 23/25 band

23-25 outer surface protein C (OspC), specific for Bb 

28 unknown

30 unknown; probably an outer surface protein; common in European and 

one California strain - Has cross-reactivity with several different types of viruses

31 outer surface protein A (OspA), specific for Bb - Has cross-reactivity with several different types of viruses

34 outer surface protein B (OspB); specific for Bb

35 specific for Bb

37 specific for Bb

38 cross-reactive for Bb

39 is a major protein of Bb flagellin; specific for Bb

41 flagellin protein of all spirochetes; this is usually the first to appear after a spirochete infection but is NOT specific to Lyme (i.e, other spirochete diseases have flagellas - see link above "Western Blot Made Easy" for more info)

45 cross-reactive for all Borellia 

50 cross-reactive for all Borrellia

55 cross-reactive for all Borrellia

57 cross-reactive for all Borrellia

58 unknown but may be a heat-shock Bb protein

60 cross reactive for all Borrellia

66 cross-reactive for all Borrelia, common in all bacteria 

83 specific antigen for the Lyme bacterium, probably a cytoplasmic membrane

93 unknown, probably the same protein in band 83, just migrates differently in some patients[/b]

 

4) Time for Antibody Response:

It takes 4 weeks minimum to develop IgM antibody response after a tick bite, and 6-8 weeks to develop IgG antibody response. The half-life of the IgM response is 5 days; this antibody is gone within a month if not reactivated by new or continuing infection.

Bands 31 and 34 take a year to develop after infection. They are prominent in chronic Lyme and in re-bites. They may appear very quickly in re-bites.