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Showing results for tags 'western blot'.
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Thank you for taking the time to read my post. Since December 17th, 2016, suddenly I have been experiencing episodes of intense anxiety, depression, depersonalization, disassociation, panic etc. These usually happen in the evening, every other day, and last for awhile (from hours to days). At the same time, there is a lot of brain fog, fatigue, and I just know there's something not right in my head, mentally. And I am very certain that it is caused by something physical (hence, why I did lyme testing). I don't have any physical symptoms such as joint aches, but in the beginning I had a constant back of the skull pressure type of headache. Recently, I took a blood test for Lyme. Here are my results. ELISA: 0.92, which is in the EQUIVOCAL range. Western Blot, Reactive Bands: 41, 58, 66, 93 (IgG) and 41 (IgM) Right now, my doctor is saying to take doxycycline 100mg twice daily for 30 days. Then after the 30 days, I will do a second western blot. Do you guys have any advice what steps I should do from here? Thanks again. REFERENCE RANGE: <=0.90 INTERPRETIVE CRITERIA: <= 0.90 NEGATIVE 0.91-1.09 EQUIVOCAL >= 1.10 POSITIVE The use of purified VlsE-1 and PepC10 antigens in this assay provides improved specificity compared to assays that utilize whole cell lysates of B. burgdorferi, the causative agent of Lyme disease, and slightly better sensitivity compared to the C6 antibody assay. As recommended by the Food and Drug Administration (FDA), all samples with positive or equivocal results in a Borrelia burgdorferi antibody EIA (screening) will be tested using a blot method. Positive or equivocal screening test results should not be interpreted as truly positive until verified as such using a supplemental assay (e.g., B. burgdorferi blot). The screening test and/or blot for B. burgdorferi antibodies may be falsely negative in early stages of Lyme disease, including the period when erythema migrans is apparent. Test Performed at: Focus Diagnostics, Inc. 33608 Ortega Highway San Juan Capistrano, CA 92675-2042 H J Batterman MD REFERENCE RANGE: NEGATIVE As per CDC criteria, a Lyme disease IgG immunoblot must show reactivity to at least 5 of 10 specific borrelial proteins to be considered positive; similarly, a positive Lyme disease IgM immunoblot requires reactivity to 2 of 3 specific borrelial proteins. Although considered negative, IgG reactivity to fewer specific borrelial proteins or IgM reactivity to only 1 protein may indicate recent B. burgdorferi infection and warrant testing of a later sample. A positive IgM but negative IgG result obtained more than a month after onset of symptoms likely represents a false-positive IgM result rather than acute Lyme disease. In rare instances, Lyme disease immunoblot reactivity may represent antibodies induced by exposure to other spirochetes. Test Performed at: Focus Diagnostics, Inc. 33608 Ortega Highway San Juan Capistrano, CA 92675-2042 H J Batterman MD
My 10 y/o son was diagnosed with Pandas in May. His symptoms have greatly improved with antibiotic treatment. We recently tested for Lyme (no symptoms yet) and received his results yesterday. I don't talk with his Dr until next week so I was wondering if anyone can give me a heads up as to what the results mean and what sort of treatment I can expect? IgM results **23-25 kDa = Ind **31 kDa = ++ **34 kDa= Ind **39 kDa= Ind **41 kDa=+ **83-93 kDa= + All non-double starred bands were negative. IgG results **41 kDa=+ All other bands were negative. As I think back over the sequence of events, I'm wondering if he could have had Lyme/Strep at the same time. He was in a wooded area the day before Thanksgiving 2012. The next day (Thursday) he got sick...said his whole body ached, hard to walk, stomach ache, headache, etc. He was diagnosed with strep on Saturday. If he had been bitten by a tick would the symptoms have hit him overnight or does it take some time? He missed a week of school but after a round of zithromax he was fine. He wasn't diagnosed with Pandas until May. If he had Lyme could that have suppressed/messed up his immune system so that the strep then caused Pandas? I realize I may be stretching for an explanation here but I'm trying to make sense of everything. I don't remember any other time in which he was bitten by a tick or had a rash. Thanks for your help!