A team of researchers at City of Hope has developed a screening assay that can quickly assess up to 1,536 compounds’ effect on estrogen activity in the body. The test can also evaluate whether chemicals act as inhibitors of aromatase, an enzyme linked to breast cancer that converts androgen to estrogen.
The results verifying this novel screening method—called AroER tri-screenTM—are published ahead of print online in Toxicological Sciences. The test can screen for more than 1,500 compounds with each run, enabling scientists to rapidly assess and identify the estrogen-disrupting impact of common chemicals. Even everyday chemicals have the potential to increase, or potentially decrease, the risk of breast cancer, researchers say.
“Approximately 70 percent of breast cancers are sensitive to estrogen, and exposure to estrogen-disrupting compounds — especially during the critical periods of pregnancy, childhood and adolescence — can have an irreversible impact on still-developing bodies,” said Shiuan Chen, Ph.D., professor and chair of City of Hope’s Department of Cancer Biology and lead author of the study. “Thus, it makes sense to develop this test, which can assess many chemicals at once, to help us quickly identify which environmental compounds are disrupting estrogen functions.”
Among other findings, the researchers found that the antidepressant paroxetine (Paxil) acted as an estrogen promoter.
“The paroxetine finding helps explain previous studies showing that it reduces tamoxifen therapy’s effectiveness,” Chen said. “And it has implications for patients with estrogen-sensitive breast cancer who are on other medications.”
Given these promising findings, Chen said that he hopes the AroER tri-screen assay will be be used to assess the estrogen-disrupting properties of more compounds, including medications, environmental pollutants and common household chemicals.
Press release: City of Hope, Duarte, California February 18, 2014. City of Hope is a leading research and treatment center for cancer, diabetes and other life-threatening diseases.